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OBJECTIVE: To analyze the variation of the anticancer drug list in the National Essential Medicines List (NEML-2018) and compare with the WHO Essential Model List (WHO-EML-2017) considering the epidemic characteristics and current national conditions, in order to provide the reference and advice for the revision of NEML in the future. METHODS: The category, content, formulation, dosage forms, medicines for children and indication for use of anticancer drug list between the NEML-2018 and WHO-EML-2017 were compared, and the deficiency existing in the NEML-2018 was analyzed. RESULTS: Compared with the NEML-2012, the category and content of the anticancer drug list have been improved in the 2018th edition, which is more reasonable for clinical request. However, disadvantages still exist in the aspect of the content, formulation, dosage forms, medicines for children and indication for use compared with the WHO-EML-2017. CONCLUSION: Although the rationality of the category and content has been improved in the NEML-2018, it still needs to be further improved compared with the WHO-EML-2017, which provides guidance and indication for the revision of anticancer drug list of the NEML in the future.
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OBJECTIVE: To explore the effects of human A30P mutant α-synuclein (α-syn) on autophagy lysosome pathway and ubiquitin proteasome system. METHODS: Constructing stable cell lines expressing human A30P mutant α-syn in PC12 cells, and detecting the levels of autophagy related proteins LC3-II and p62 as well as ubiquitinated proteins. Furthermore, the influence of A30P mutant α-syn on cell viability upon normal and stress conditions was conducted by MTT assay. RESULTS: Expressing A30P mutant α-syn could significantly reduce autophagy related protein LC3-II level and increase level of autophagy substrate p62, as well as promote aggregation of ubiquitinated proteins. Otherwise, expressing A30P mutant α-syn did not reduce cell viability under normal conditions. However, cell viability was significantly reduced under treatment with neurotoxin rotenone or serum free condition in A30P mutant α-syn groups compared with empty vector group. CONCLUSION: Over expression of human A30P mutant α-syn could impair the protein degradation system and increase the sensitivity of cells to toxic insults.
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<p><b>OBJECTIVE</b>The composite membrane was made by recombinant human bone morphogenetic protein-2 (rhBMP-2), collagen (Co), polylactic-co-glycolic acid (PLGA). To observe the ectopic bone formation and evaluate the capability of rhBMP-2/Co/PLGA complex membrane.</p><p><b>METHODS</b>48 male Kunming adult mice were divided into two groups randomly: Co/PLGA complex membrane group and rhBMP-2/Co/PLGA complex membrane group. After etherized, an incision was made on the outer muscle and two groups of complex membranes were implanted into the right side hind limb muscle pouch. 8 mice from each group were sacrificed at 7, 14, 28 d. The hind limbs were removed, examined by soft X-ray, HE staining and light microscope examination.</p><p><b>RESULTS</b>Wounds healed well, no infections and rejections were observed. In the Co/PLGA group, partial implanted membrane degradated and absorbed at 7 d. The membrane collagen fiber appeared loose at 14 d. The membrane lost its intact to disrupted at 28 d. No ectopic bone formation was found. In the rhBMP-2/Co/PLGA group, ectopic bone formation was found by eye, soft X-ray and histological examinations. The rhBMP-2/Co/PLGA complex membrane degraded slower than Co/PLGA complex membrane.</p><p><b>CONCLUSION</b>Co/PLGA can provide a carrier for rhBMP-2 to have stronger effect of biological activity.</p>